簡要描述:Illumina 簇生成和測序試劑 RS-122-2303上海易匯生物科技有限公司于17年正式銷售Illumina公司基因芯片、二代測序NGS測序儀及相關測序試劑盒等產品Illumina/TruSeq Stranded Total RNA Library Prep Kit with Ribo-Zero Gold High Throughput (96 samples, 96 indexes)
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品牌 | illumina/美國因美納 | 貨號 | RS-122-2303 |
---|---|---|---|
規格 | 1 kit | 供貨周期 | 兩周 |
主要用途 | 小型全基因組測序 組合試劑 試劑盒 | 應用領域 | 醫療衛生,化工,生物產業,制藥 |
Illumina 簇生成和測序試劑 RS-122-2303
"Illumina/TruSeq Stranded Total RNA Library Prep Kit with Ribo-Zero Gold High Throughput (96 samples, 96 indexes)/RS-122-
" Illumina 產品編號: RS-122-2303美 元 價: $10280.00會 員 價: 待定品 牌: Illumina產 地: 美國公 司: Illumina, Inc.產品分類: 分子類>二代測序>DNA文庫制備試劑盒公司分類: Library Preparation Kits "Product Highlights:
The TruSeq Stranded Total Library Preparation kit provides a clear view of the transcriptome with a streamlined, cost-efficient, and scalable solution for total RNA analysis with sequencing.
上海易匯生物科技有限公司于17年正式銷售Illumina公司基因芯片、二代測序NGS測序儀及相關測序試劑盒等產品
咨詢illumina MiSeq測序試劑盒等產品歡迎您致電 上海易匯生物科技有限公司:1850 1609 238張經理
"Illumina/Nextera DNA CD Indexes (24 Indexes, 24 Samples)/20018707/1 Ea
" Illumina 產品編號: 20018707美 元 價: $120.00會 員 價: 待定品 牌: Illumina產 地: 美國公 司: Illumina, Inc.產品分類: 分子類>二代測序>DNA文庫制備試劑盒公司分類: Library Preparation Kits "Product Highlights:
The Nextera DNA Flex Library Prep Kit offers flexibility for many whole-genome sequencing applications.
Fastest Illumina library prep workflow, with ~3.5 hours total time
Flexibility to accommodate variations in sample type, DNA input amount, and application
Optimized library prep performance, generating reliable results
Save Time and Resources
The Nextera DNA Flex Library Prep Kit uses a fast, user-friendly workflow. On-bead tagmentation chemistry reduces total library prep time to ~3.5 hours, from DNA extraction to library normalization.
Simplify Lab Operations
The Nextera DNA Flex workflow supports a broad DNA input range (1–500 ng), multiple sample types, and both small and large genomes. The workflow includes DNA extraction from blood, saliva, or dried blood spots*or bacterial colonies*. Gain the flexibility to sequence human or other large, complex genomes as well as amplicons or microbial species, all with a single kit.
Obtain Reliable Results
While accommodating various study requirements, the Nextera DNA Flex workflow delivers consistent insert sizes, uniform coverage, and optimized performance, regardless of DNA input amount or genome size. The bead-based technology minimizes bias and opportunities for error, resulting in highly reproducIBLe sequencing data.
*Demonstrated protocols available.
Specifications:
Assay Time ~3.5 hours total assay time
Hands-On Time 1-1.5 hours
Input Quantity Small Genomes: 1-500 ng DNA; Large Genomes: 100-500 ng
Content Specifications Human Whole Genome, Small Whole Genome, Large Whole Genome
Mechanism of Action Bead-linked transposome
Multiplexing Up to 96 available indexes
Species Category Any Species
System Compatibility MiSeq,HiSeq X Ten,NextSeq 550,HiSeq 3000,HiSeq X Five,MiSeqDx in Research Mode,MiniSeq,HiSeq 2500,NovaSeq 6000,HiSeq 4000
Variant Class Single Nucleotide Polymorphisms (SNPs),Gene Fusions,Loss of Heterozygosity (LOH),Somatic Variants,Chromosomal Abnormalities,Germline Variants,Structural Variants,Insertions-Deletions (indels),Copy Number Variants (CNVs)
Method Whole-Genome Sequencing
Technology Sequencing
Automation CapABIlity Liquid Handling Robots"
illumina FC-410-1003 HiSeq 3000/4000 SBS Kit (300 cycles)
illumina FC-420-1001 MiniSeq High Output Reagent Kit (75-cycles)
illumina FC-420-1002 MiniSeq High Output Reagent Kit (150-cycles)
illumina FC-420-1003 MiniSeq High OutputReagent Kit (300-cycles)
illumina FC-420-1004 MiniSeq Mid Output Kit (300-cycles)
illumina FC-451-1001 ForenSeq Index Adapter Fixture
illumina FC-501-2501 HiSeq X Ten Reagent Kit v2.5
illumina FC-501-2521 HiSeq X Ten Reagent Kit v2.5 - 10 pack
illumina FC-502-2501 HiSeq X Five Reagent Kit v2.5
illumina FC-502-2521 HiSeq X Five Reagent Kit v2.5 - 10 pack
illumina FC-901-1001 Hybridization Manifold (0801-1320)
illumina FC-901-1002 Amplification Manifold (0801-1321)
illumina FC-901-1003 Cs, Bridge Manifold
illumina Nextera DNA Sample Preparation Kits
illumina FC-121-1031/1030/1012/1011
產品名稱:llumina MiSeq Reagent Kit v2 (500-cycles)基因測序試劑盒
產品貨號:MS-102-2003
規格:
大輸出 8.5 Gb(500個循環數MiSeq Reagent Kit v2)、5.1 Gb(300個循環數MiSeq Reagent Kit v2)、1.2 Gb(300個循環數MiSeq Reagent Micro Kit v2)、0.850 Gb(50個循環數MiSeq Reagent Kit v2)、0.5 Gb(500個循環數MiSeq Reagent Nano Kit v2)、0.3 Gb(300個循環數MiSeq Reagent Nano Kit v2)
每次運行獲得的大read數 多達1500萬
核酸類型 DNA, RNA
試劑類型 簇生成, 雙端測序, 邊合成邊測序
技術 測序
系統兼容性 研究模式下的MiSeqDx ,研究模式下的MiSeq FGx, MiSeq , MiSeq
產品介紹:
MiSeq試劑盒v2實現更高通量,減少MiSeq運行次數。MiSeq試劑盒v2保留了與v1試劑盒相同的即用型預裝試劑盒,但改良了化學過程,提高簇密度,減少循環時間,并提高質量(Q)分值。目前還提供Micro和Nano規格的MiSeq試劑盒v2,適合低通量的應用。
改良化學過程后,循環時間更快、片段更長、輸出更多
雙表面成像實現的片段數是v1試劑盒單表面成像的兩倍
使用500次循環的試劑盒可擴展片段長度
選擇適合您應用的循環次數(50、300或500)
與升級的MiSeq系統結合使用,MiSeq試劑盒v2的雙表面成像可使每個流動槽的通量翻倍。MiSeq試劑盒v2提供可生成較長片段長度的500次循環格式,以及廣受歡迎的50次循環和300次循環格式。
MiSeq試劑盒v2有標準、Micro和Nano三種配置,能滿足特定項目需求和可擴展輸出量。
所有MiSeq試劑組件均采用RFID編碼,并與MiSeq系統智能交互,以驗證與用戶定義的應用程序的兼容性。
一個MiSeq試劑盒v3的也可以。它提供600循環格式,允許任何Illumina測序系統具有長的讀取長度,或150循環格式,可實現計數應用。
該產品也可作為Illumina Advantage(TG)產品提供。Illumina Advantage大規模測序產品具有特定批次的出貨和測試,延長的保質期和*的變更通知,可提高實驗室效率。
Illumina公司簡介:
Illumina公司是由David Walt博士、CW 集團的Larry Bock、獸醫學博士John Stuelpnagel、 Anthony Czarnik博士及Mark Chee博士于1998年4月共同組建,是遺傳變異和生物學功能分析領域的優秀的產品、技術和服務供應商。通過幫助客戶加快實現生物信息的采集、分析和應用,來改善人類健康。
背景知識:
全基因組測序概述
全基因組測序可謂是基因組zui為全面的研究方案?;蚪M信息已能用于鑒定遺傳疾病,查找驅使癌癥發展的突變,追蹤疾病的爆發。illumina迅速下降的測序成本以及處理大樣本數據能力的提升都使得如今的測序者可將全基因組測序視為基因組研究的zui強有力工具。
全基因組測序常被理解為用于測定人類基因組,然而illumina新一代測序技術(NGS)的規模、靈活性體現于可以在任何物種上運用測序技術,如農業畜牧業,植物,或疾病相關微生物。
illumina全基因組測序的優點
提供高分辨率、到逐個堿基的基因組視圖
可捕獲大的變異,以及小到可能被遺漏的變異
鑒定潛在的致病變異,從而進行基因表達和調控機制的進一步研究
在短時間內提供大量的數據,以支持新基因組的組裝
呈現基因組視圖
外顯子組測序或靶向重測序等有側重點的方法只分析基因組的有限部分,全基因組測序則不同,能提供整個基因組的全面視圖。它是各種發現應用——如鑒定致病變異和新基因組組裝——的理想選擇。全基因組測序可檢測單核苷酸變異、插入/缺失、拷貝數改變和大的結構變異。隨著技術創新,的基因組測序儀能夠比以往更地開展全基因組測序。
illumina主要的全基因組測序方法
大型全基因組測序
測序大型基因組(> 5 Mb),如人類、植物或動物基因組,為疾病和群體遺傳學研究提供寶貴信息。
小型全基因組測序
小型基因組測序(≤ 5 Mb)包括測序細菌、病毒或其他微生物的整個基因組。無需培養細菌,研究人員可利用NGS平行測序數千個小生物。
De Novo 測序
De novo測序是指在沒有參考序列的情況下,對一個新的基因組進行測序。NGS能實現對任何物種的快速、準確鑒定。
Phased基因組測序
Phased測序,又稱基因組Phased。通過區分同源染色體的等位基因差異而獲得全基因組單體型,該信息通常對遺傳疾病的研究至關重要。
產品名稱:illumina MiSeq基因測序試劑盒v2 (500循環) 產品貨號:MS-102-2003
MiSeq Reagent Kits v2系列產品:
品牌 產品貨號 產品名稱
illumina品牌 MS-102-2001 MiSeq Reagent Kit v2 (50-cycles)基因測序試劑盒
illumina品牌 MS-102-2002 MiSeq Reagent Kit v2 (300-cycles)基因測序試劑盒
illumina品牌 MS-102-2003 MiSeq Reagent Kit v2 (500-cycles)基因測序試劑盒
illumina品牌 MS-102-2021 20-pack MiSeq Reagent Kit v2 (50-cycles)基因測序試劑盒
illumina品牌 MS-102-2022 20-pack MiSeq Reagent Kit v2 (300-cycles)基因測序試劑盒
illumina品牌 MS-102-2023 20-pack MiSeq Reagent Kit v2 (500-cycles)基因測序試劑盒
illumina品牌 MS-103-1001 MiSeq Reagent Nano Kit v2 (300-cycles)基因測序試劑盒
illumina品牌 MS-103-1002 MiSeq Reagent Micro Kit v2 (300-cycles)基因測序試劑盒
illumina品牌 MS-103-1003 MiSeq Reagent Nano Kit v2 (500-cycles)基因測序試劑盒
illumina品牌TruSight系列相關產品:
品牌 產品貨號 產品名稱
illumina品牌 15065075 TruSight? (12 samples) Rapid Capture Reagents 2 to 8°C
illumina品牌 15065076 TruSight? (12 samples) Rapid C
illumina品牌 15065078 TruSight? Cardio (12 samples)Oligos
illumina品牌 20000215 TruSight HLA v2 Sequencing Panel (24 samples)
illumina品牌 20005170 TruSight HLA v2 Sequencing Panel (24 samples, automated)
illumina品牌 20005610 TruSight Tumor 15 MiniSeq Kit
illumina品牌 20005612 TruSight Cancer MiniSeq Kit
illumina品牌 20006993 TruSight HLA (24 Samples) Box 3 of 4 Post-PCR
illumina品牌 20006994 TruSight HLA (24 Samples) Box 4 of 4 Post-PCR
illumina品牌 20006995 TruSight HLA Boxes 3 and 4
illumina品牌 20010179 TruSight HLA-B Primers v2
illumina品牌 20010187 TruSight? Oncology Enrichment
illumina品牌 20010188 TruSight? Tumor 170 Content Set
illumina品牌 20010190 TruSight HLA-DRB Primers v2
illumina品牌 20014226 GRAIL HT TruSight? LP Kit
illumina品牌 20014227 GRAIL HT TruSight? Enrichment Kit
illumina品牌 20015966 TruSight Lymphoma 40 (8 Samples)
illumina品牌 20015967 TruSight Lymphoma 40 (48 Samples)
illumina品牌 20015968 TruSight? Acute Lymphoblastic Leukemia Consortia Panel 52 (16 Samples)
illumina品牌 20015969 TruSight? Acute Lymphoblastic Leukemia Consortia Panel 52 (96 Samples)
illumina品牌 20015970 TruSight Myeloma 43 (16 Samples)
illumina品牌 20015971 TruSight Myeloma 43 (96 Samples)
illumina品牌 20018621 TruSight Tumor 170 Kit, For Use with NextSeq plus Watson for Genomics
illumina品牌 20018622 TruSight Tumor 170 Kit plus Watson for Genomics
illumina品牌 FC-121-0202 TruSight Cancer
illumina品牌 FC-121-0205 TruSight Inherited Disease Sequencing Panel (48 samples)
illumina品牌 FC-123-1203 TruSight Enrichment Kit (24 Indices - 48 Samples)
illumina品牌 FC-123-1205 TruSight Enrichment Kit (24 Indices - 96 Samples)
illumina品牌 FC-130-1010 TruSight Myeloid Sequencing Panel (96 samples)
illumina品牌 FC-130-2001 TruSight Tumor 26
illumina品牌 FC-141-1006 TruSight One Sequencing Kit (9 samples)
illumina品牌 FC-141-1007 TruSight One Sequencing Panel (36 samples)
illumina品牌 FC-141-2007 TruSight One Expanded Sequencing Panel (36 samples)
illumina品牌 FC-142-1001 TruSight? HLA Sequencing Panel (24 samples)
illumina品牌 OP-101-1001 TruSight Tumor 15 MiSeq Kit
illumina品牌 OP-101-1003 TruSight Tumor 170 Kit, For Use with NextSeq (24 Samples)
illumina品牌 OP-101-1004 TruSight Tumor 170 Kit (24 Samples)
illumina品牌 TG-130-2001 TG TruSight Tumor (48 Samples)
illumina品牌 TG-140-1102 TG TruSight Rapid Capture (2 Indices, 8 Samples)
illumina品牌 TG-140-1103 TG TruSight Rapid Capture (4 Indices, 16 Samples)
illumina品牌 TG-140-1104 TG TruSight Rapid Capture (24 Indices, 48 Samples)
illumina品牌 TG-140-1105 TG TruSight Rapid Capture (24 Indices, 96 Samples)
illumina品牌 TG-140-1106 TG TruSight Rapid Capture (96 Indices, 288 Samples)
illumina品牌 TG-141-1002 TG TruSight Cancer Sequencing Panel
illumina品牌 TG-141-1005 TG TruSight Inherited Disease Sequencing Panel (48 Samples)
illumina品牌 TR-203-0015 TruSight Tumor Sample Preparation
Whole-Transcriptome Analysis with Precise Measurement of Strand Orientation
CompatIBLe with a wide range of samples, including low-quality and FFPE, TruSeq Stranded Total RNA kits couple all of the benefits of TruSeq RNA preparation kits with Ribo-Zero ribosomal RNA reduction chemistry, providing analysis of coding and multiple forms of noncoding RNA with precise measurement of strand orientation, uniform coverage, and high-confidence discovery of features such as alternative transcripts, gene fusions, and allele-specific expression.
Find an up-to-date list of high-throughput automation vendors with robotic systems compatIBLe with this library preparation kit
FlexIBLe Solutions for a Range of Study Designs
The TruSeq Stranded Total RNA Library Prep kit is available in multiple versions to fit specific study design needs:
TruSeq Stranded Total RNA with Ribo-Zero Human/Mouse/Rat removes cytoplasmic rRNA.
TruSeq Stranded Total RNA with Ribo-Zero Gold removes both cytoplasmic and mitochondrial rRNA.
The total RNA kits are also compatIBLe with the new unique dual index kits, TruSeq RNA Unique Dual Indexes, which allows multiplexing of up to 96 samples.
Species Compatibility
The TruSeq Stranded Total Library Preparation kit is designed for human, mouse and rat sequences, but may be applicable to a variety of eukaryotic species.*
*For information on a particular species of interest, contact Illumina Technical Support.
Specifications:
Input Quantity 0.1 – 1 ug high-quality total RNA. Lower-quality samples may require further optimization.
Content Specifications Captures coding RNA plus multiple forms of non-coding RNA
Mechanism of Action Bead-based rRNA depletion, CDNA synthesis, and PCR
Multiplexing Low-throughput kits: Pool up to 12 samples. Or pool up to 24 samples with sets A and B together,High-throughput kits: Prepare 96 uniquely indexed samples.
System Compatibility NovaSeq 5000,Genome Analyzer IIx,HiSeq 2000,NextSeq 550,HiSeq 3000,HiSeq 1000,HiSeq 1500,NextSeq 500,HiSeq 2500,NovaSeq 6000,HiSeq 4000
Variant Class Single Nucleotide Polymorphisms (SNPs),Gene Fusions,Novel Transcripts,Transcript Variants
Specialized Sample Types FFPE
Method Whole-Transcriptome Sequencing
Species Category Mouse,Human,Rat
Technology Sequencing
Automation CapABIlity Liquid Handling Robots"
Illumina公司,致力于新一代測序和芯片技術的生產與開發,提供產品與應用資訊
Illumina公司創立于1998年4月,是遺傳變異和生物學功能分析領域的優秀的產品、技術和服務供應商。通過幫助客戶加快實現生物信息的采集、分析和應用,來改善人類健康。
Sequencing Kits/ Microarray Kits/Informatics Products/ Clinical Research Products/In Vitro Diagnostic/ Products/Molecular Biology Reagents/ Accessory Products
上海易匯生物科技有限公司于17年正式銷售Illumina公司基因芯片、二代測序NGS測序儀及相關測序試劑盒等產品
咨詢illumina MiSeq測序試劑盒等產品歡迎您致電 上海易匯生物科技有限公司:1850 1609 238張經理
Illumina 簇生成和測序試劑 RS-122-2303
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